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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: Efficient bioproduction of 5-aminolevulinic acid, a promising biostimulant and nutrient, from renewable bioresources by engineered Corynebacterium glutamicum

Fig. 6

Fed-batch fermentation for 5-ALA production using different carbon sources. Black square, OD600nm; green circle, 5-ALA. Dotted line represents simulated growth curve based on OD600nm data. Cultivation was performed in 5-L bioreactors with fermentation medium. IPTG (0.1 mM) and glycine (4 g/L) were added when OD600nm reached approximately 40 to induce gene expression and 5-ALA biosynthesis. Sugar solution and glycine were continuously fed into the bioreactor during the fermentation. The pH was controlled at 6.5 initially and switched to 6.0 at 15 h. The dissolved O2 was maintained at 30% initially and switched to 10% at 18 h

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