Table 2 Strains and plasmids used in this study

Strain

 E. coli DH5α

F⁻supE44 ∆lacU169 (φ80 lacZ∆M15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1

Invitrogen

 C. glutamicum ATCC 13032

Wild type

ATCC

 CA

C. glutamicum ATCC 13032 derivative harboring plasmid pRpA

This study

 CO

C. glutamicum ATCC 13032 derivative harboring plasmid pRpO

This study

 CA-Rs

C. glutamicum ATCC 13032 derivative harboring plasmid pRsA

This study

 CA1

C. glutamicum ATCC 13032 derivative harboring plasmid pRpA1

This study

 CA2

C. glutamicum ATCC 13032 derivative harboring plasmid pRpA2

This study

 CA3

C. glutamicum ATCC 13032 derivative harboring plasmid pRpA3

This study

 CA4

C. glutamicum ATCC 13032 derivative harboring plasmid pRpA4

This study

 CA1P

C. glutamicum ATCC 13032 derivative harboring plasmid pRpA1P

This study

 CA1P1

C. glutamicum ATCC 13032 derivative harboring plasmid pRpA1P1

This study

 CA1P2

C. glutamicum ATCC 13032 derivative harboring plasmid pRpA1P2

This study

 CA1P3

C. glutamicum ATCC 13032 derivative harboring plasmid pRpA1P3

This study

 CA1P4

C. glutamicum ATCC 13032 derivative harboring plasmid pRpA1P4

This study

 CA1P5

C. glutamicum ATCC 13032 derivative harboring plasmid pRpA1P5

This study

 CA1P6

C. glutamicum ATCC 13032 derivative harboring plasmid pRpA1P6

This study

Plasmid

 pET28a-RS-hemA

pET28a harboring hemA from R. sphaeroides (RsHemA), Kan^R

[52]

 pEC-XK99E

E. coli–C. glutamicum shuttle vector, P_trc promoter, Km^R

[58]

 pRpA

pEC-XK99E harboring hemA from R. palustris ATCC 17001 (RpHemA)

This study

 pRpO

pEC-XK99E harboring hemO from R. palustris ATCC 17001 (RpHemO)

This study

 pRsA

pEC-XK99E harboring hemA from R. sphaeroides (RsHemA)

This study

 pRpAtuf

pRpA derivative, P_trc promoter of RpHemA replaced with P_tuf promoter of C. glutamicum ATCC 13032

This study

 pRpAsod

pRpA derivative, P_trc promoter of RpHemA replaced with P_sod of C. glutamicum ATCC 13032

This study

 pRpA1

pRpA derivative, RBS-1 of RpHemA replaced with RBS-2

This study

 pRpA2

pRpA derivative, RBS-1 of RpHemA replaced with RBS-3

This study

 pRpA3

pRpA derivative, RBS-1 of RpHemA replaced with RBS-4

This study

 pRpA4

pRpA derivative, RBS-1 of RpHemA replaced with RBS-5

This study

 pRpA1P

pRpA1 derivative, harboring ppc from C. glutamicum ATCC 13032

This study

 pRpA1P1

pRpA1P derivative, RBS-1 of ppc replaced with RBS-2

This study

 pRpA1P2

pRpA1P derivative, RBS-1 of ppc replaced with RBS-6

This study

 pRpA1P3

pRpA1P derivative, RBS-1 of ppc replaced with RBS-7

This study

 pRpA1P4

pRpA1P derivative, RBS-1 of ppc replaced with RBS-8

This study

 pRpA1P5

pRpA1P derivative, RBS-1 of ppc replaced with RBS-5

This study

 pRpA1P6

pRpA1P derivative, RBS-1 of ppc replaced with RBS-9

This study