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Table 2 The kinetic parameters of free and immobilized ROL

From: Lipase immobilized on functionalized superparamagnetic few-layer graphene oxide as an efficient nanobiocatalyst for biodiesel production from Chlorella vulgaris bio-oil

  Km (mM) Vmax (µM min−1) kcat (min−1) kcat/Km (min−1 mM−1)
ROL 16.45 ± 0.46 541.52 ± 26.68 115.22 ± 7.94 7.01
ROL/MNP 12.25 ± 0.54 212.77 ± 11.11 45.26 ± 4.26 3.69
ROL/MGO 12.67 ± 0.62 336.98 ± 12.42 71.69 ± 6.35 5.91
ROL/MGO–AP 4.01 ± 0.22 357.23 ± 8.24 76.55 ± 5.18 18.95
ROL/MGO–AP–GA 3.04 ± 0.18 369.12 ± 9.83 78.53 ± 6.76 25.83