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Table 3 Lactococcus lactis strains and plasmids used in this study

From: Synergy at work: linking the metabolism of two lactic acid bacteria to achieve superior production of 2-butanol

Designation

Genotype or description

References

Strains

  

 CS4363

MG1363 Δ3ldh Δpta ΔadhE

[22]

 MM01

CS4363 pButop pDdrAB

This work

 MM10

CS4363 pButop

This work

 MM06

CS4363 pMM06

This work

Plasmids

  

 pTD6

E. coli/L. lactis shuttle vector containing gusA reporter, Tet

[22]

 pJM001

pTD6::bdh, Tet

[43]

 pCI372

E. coli/L. lactis shuttle vector, Cam

[44]

 pButop

pCI372::pddABC-sadB

This work

 pDdrAB

pTD6::ddrAB

This work

 pMM06

pTD6::bdh-sadB

This work