Fig. 1

Growth phenotypes of strains ORCB001 and ORCB002 when grown in increasing amounts of added glycerol. Glycerol was added to make total initial (calculated) media osmolarities of a 400 mOsm/L, b 500 mOsm/L, and c 600 mOsm/L. Strain JWCB005 is the genetic parent strain to both single-gene deletion strains. Growth assays were done in 50 mL culture volumes in 135 mL serum bottles containing a headspace of 100% N₂