Fig. 5

Growth phenotypes of strains ORCB011, ORCB012, and ORCB013 when grown in a added glycerol to total starting osmolarity of 500 mOsm/L, and b added NaCl to total starting osmolarity of 300 mOsm/L. The genetically complemented fruR/cra-deletion strain ORCB012 exhibited shorter lag phases and increased growth rates relative to an empty vector control (ORCB011), suggesting osmolyte phenotypes observed in ORCB002 are not due to the absence of the fruR/cra gene, but instead from other unintended genomic differences between ORCB002 and its genetic parent strain JWCB005