Gene targets for engineering osmotolerance in Caldicellulosiruptor bescii

Table 6 Genes that were found to have similar differential expression in strains ORCB001 and ORCB002, each compared to their common genetic parent strain JWCB005

Locus tag	Annotation	Average log₂(ORCB002/JWCB005) across three osmolarity conditions	Average log₂(ORCB001/JWCB005) across three osmolarity conditions
B5X54_RS00980	Endo-1,4-beta xylanase	− 4.1	− 4.2
B5X54_RS05305	Hypothetical	− 4.9	− 2.5
B5X54_RS05310	Hypothetical	− 4.3	− 2.4

Values are average differential log-fold changes observed for the indicated gene across three indicated high-osmolarity conditions (glucose, glycerol, sodium chloride). As these genes were similarly differentially expressed in strains showing different growth phenotypes, it is unlikely these genes are contributing to observed phenotype differences, and were not considered in this study. All values are statistically significant (Wald test, Benjamini–Hochberg adjusted p value < 0.05, n = 3)

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