Table 2 Genes and primers used for qRT-PCR
Gene | Forward primer sequence (5′–3′) | Reverse primer sequence (5′–3′) |
|---|---|---|
MidA | GAGTCCGACCAAACAATAAAATCA | CATCGCCATTTGCCGTAAG |
CchA | AGGTTATCCTCCGCAGTCTCAA | CGATAGCAAACAGAAGCCAGAA |
CrzA | TGCTCTGGGTCGTCATTTCC | CCCGCTCTTGAGACTCTTCATC |
CnaA | GTCGGCACTTGACGGACTACTT | GGCAAGGAGCAAAACGATTC |
RodA | CCGCTGCTGTTCTTGCTTTC | TTGCCATCGTTGCCGTTAC |
PksA | CGAACGTGAGCTTGAAAGGAT | ACTCGGTGTTTTCACGCTCTTC |
ChsA | AAGGATGGTTGGAAGAAGATTGTG | CAAAGCCGCCAAAGCATT |
ChsB | CCGAGGACCGTATCTTGTGTTT | CAGTTTCACCCTTTGAGGCTTT |
ChsC2 | AGTTTATTCTTCAGCGTCGTCGTT | CGCTCCGCCAAATCTGATAG |
ChsD | ATTCAAGCACGGTGGCAAAC | GTCGGCATAAACCTTGTCAATCA |
ChsC1 | TCGTGGTCTGTTTGGTTTTCG | ATCGTCGCCAACACATCCA |
Actin | GGTCTGGAGAGCGGTGGTAT | GAAGAAGGAGCAAGAGCAG |
ags1 | ACAACACGAACCGCACCAT | CGGGTGGAACAGGTTTTTGA |
ags2 | TGCTGCGTGCGTTTATGC | TCGTGGATTGGCCGTCTT |
ags3 | TGGGAGGGCACTCACATTC | GCGAGGGACACCGGATTC |
gtb3 | TCCATGGTCGTGATAATGTGAAG | GCCTCCATCCGTTTCATCAT |
agd3 | CTTCACCCACGAGGAGCAA | CCGTTGGCAGTGAAGTGCTT |
ega3 | GGAGCGTCAACGAGCAATG | CCGTGAAGGCAGCGTAGGT |
uge3 | GGACCTTGCGGCTTCTGAT | GGCCAGATTCGTCACATCCT |
uge5 | CCATCCGCGACTACATCCA | CAGGGCCTTCAAGTGACCAT |