Fig. 6

Substrate specificity of HaSGNH1 variants. Substrate specificity of HaSGNH1 variants toward p-nitrophenyl esters. Results of a N83L, b M86R, c M86E, d R87L, e F131A, and f I173F are shown. Results from wild-type HaSGNH1 are shown for comparison in black. In these experiments, the enzyme activity of wild-type HaSGNH1 under standard assay condition was defined as 100%. All experiments were performed at least in triplicate