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Table 1 Kinetic parameters of HaSGNH1 towards p-NP esters

From: Biodiesel and flavor compound production using a novel promiscuous cold-adapted SGNH-type lipase (HaSGNH1) from the psychrophilic bacterium Halocynthiibacter arcticus

Substrate

Vmax (s−1 μM)

KM (mM)

kcat (s−1)

kcat/KM (s−1 mM)

p-NA (C2)

0.49 (± 0.03)

0.93 (± 0.15)

8.38 (± 0.53)

8.9

p-NB (C4)

1.00 (± 0.11)

0.80 (± 0.23)

17.1 (± 1.83)

21.3

p-NH (C6)

0.96 (± 0.08)

0.99 (± 0.20)

16.5 (± 1.45)

16.7

  1. Kinetic parameters of HaSGNH1 were determined using Michaelis–Menten kinetics. Three substrates of p-NP esters (p-NA, p-NB, and p-NH) were used for comparison. Kinetic parameters (Vmax, KM, and kcat) were calculated by directly fitting to the Michaelis–Menten plot
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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