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Table 3 Kinetic parameters of HaSGNH1 and its variants toward p-NB

From: Biodiesel and flavor compound production using a novel promiscuous cold-adapted SGNH-type lipase (HaSGNH1) from the psychrophilic bacterium Halocynthiibacter arcticus

Substrate

Vmax (s−1 μM)

KM (mM)

kcat (s−1)

kcat/KM (s−1mM)

Wild type

1.00 (± 0.11)

0.80 (± 0.23)

17.1 (± 1.83)

21.3

R87L

1.15 (± 0.06)

0.68 (± 0.10)

15.0 (± 0.84)

22.2

M86R

0.78 (± 0.05)

1.26 (± 0.17)

5.1 (± 0.35)

4.2

M86E

0.61 (± 0.06)

1.06 (± 0.23)

4.0 (± 0.39)

3.8

  1. Kinetic parameters of HaSGNH1 and its four variants were determined using p-NB. Kinetic parameters (Vmax, KM, and kcat) were calculated by directly fitting to the Michaelis-Menten plot
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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