Fig. 5

Effect of esterase supplementation on xylanase hydrolysis of Japanese beechwood biomass. Evolution of reducing sugars during supplementation of Xyn11A with the F. johnsoniae enzymes (a) and the B. ovatus enzymes (b): Xyn11A (black dot), Xyn11A + CE1 domain (blue triangle), Xyn11A + CE6 domain (green square), Xyn11A + equimolar mix of CE6 and CE1 (yellow dot) and Xyn11A + full-length construct (purple triangle). Xylooligosaccharide concentrations after 7.5 h incubation with Xyn11A, supplemented with the F. johnsoniae CE enzymes (c) and the B. ovatus enzymes (d): xylose (grey), xylobiose (white), xylotriose (black) and xylotetraose (checked). Reactions with the F. johnsoniae constructs were performed at 25 °C, while the corresponding reactions containing B. ovatus constructs were carried out at 37 °C, explaining the difference in Xyn11A product release in the reactions not supplemented with esterases. Results are presented as the average of triplicate experiments with standard errors