Fig. 1
From: Biotransformation of keratin waste to amino acids and active peptides based on cell-free catalysis
Effect of promoters on keratinase activity and the enzymatic properties of recombinant keratinase KerZ1. a Schematic representation of different promoters and their corresponding keratinase activities. WT: wild-type keratinase activity secreted by Bacillus licheniformis BBE11-1. b SDS-PAGE analysis of recombinant keratinase. Lane 1: The fermentation supernatant of Bacillus subtilis WB600, Lane 2: Recombinant keratinase KerZ1, Lane 3: Purified recombinant keratinase KerZ1. c Optimum pH of KerZ1. d Optimum temperature of KerZ1. e pH stability. KerZ1 was appropriately diluted with buffers of different pH and incubated at 37 °C for 90 min, after which the residual enzyme activity of keratinase was measured at an interval of 30 min. f Temperature stability. KerZ1 was incubated at different temperatures for 90 min. The residual enzyme activity of keratinase was then measured at an interval of 30 min