Fig. 2
From: Biotransformation of keratin waste to amino acids and active peptides based on cell-free catalysis
Effects of ribosome binding on translation initiation rate and keratinase activity in the corresponding mutant. a Prediction of the keratinase translation level using the RBS Calculator v2.0 (https://salislab.net/software/forward). b Keratinase activity corresponding to RBS mutants. c Analysis of keratinase expression by SDS-PAGE. Lane 1: RBS1, Lane 2: RBS2, Lane 3: RBS3, Lane 4: RBS9, Lane 5: Keratinase expressed by a recombinant strain containing the original RBS sequence served as a control (CK). d Batch fermentation of mutant RBS3 in 15 L fermenter