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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Biotransformation of keratin waste to amino acids and active peptides based on cell-free catalysis

Fig. 3

Synergetic effect of chemical reducing agent on degradation of feathers by KerZ1. a Effect of reducing agent on keratinase-hydrolyzing feathers. a1: Feathers were treated with KerZ1 (45.14 KU/mL) at 60 °C for 4 h; a2: feathers were treated with reducing agent β-Me, sodium sulfite and dithiothreitol at a final concentration of 0.1% at 60 °C for 4 h; a3: feathers were treated with KerZ1 containing 0.1% reducing agent β-Me (β-mercaptoethanol), sodium sulfite and dithiothreose (DTT) at 60 °C for 4 h. b Feathers were treated with KerZ1 (426.60 KU/mL) at 60 °C for 12 h. c Feathers were treated with KerZ1 (426.60 KU/mL) containing 1% sodium sulfite at 60 °C for 12 h

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