Fig. 3

Anaerobic ethyl acetate production in E. coli BW25113 (DE3) and E. coli BW25113 ΔackAΔldhA expressing the Kma eat1 from pET26b:hKmaEat1. a Fermentation profile of E. coli BW25113 (DE3) (pET26b:hKmaEat1). b Fermentation profile of E. coli BW25113 ΔackAΔldhA (DE3) (pET26b:hKmaEat1). c Product yields during anaerobic ethyl acetate production. Experiments were performed as biological duplicates. Strains were grown in sealed and N₂ flushed serum bottles under anaerobic conditions in modified M9 medium at 30 °C and 150 rpm. Gene expression was induced with 0.05 mM IPTG. Error bars represent the standard deviation. Ethyl acetate concentration in the headspace, CO₂ and H₂ were not measured