Fig. 5

Comparison of truncated eat1 genes under various gene expression levels induced by 0.01–0.5 mM IPTG or 0.001–0.01 mM IPTG. a–c Fermentation product yields of strains expressing the Kma Eat1, the Kma trEat1 F-26 and the Kma trEat1 K-30, respectively, under the control of the LacI/T7 promoter after 120 h of cultivation. Gene expression was induced with 0.01–0.5 mM IPTG. d–f Fermentation product yields of strains expressing the Wan Eat1, the Wan trEat1 V-11 and the Wan trEat1 N-13, respectively, under the control of the LacI/T7 promoter after 120 h of cultivation. Gene expression was induced with 0.001–0.01 mM IPTG. Strains were grown in sealed and N₂ flushed serum bottles under anaerobic conditions in modified M9 medium at 30 °C and 150 rpm. Genes were expressed in E. coli BW25113 ΔackAΔldhA (DE3) from a series of pET26b plasmids. Succinate and formate were detected but concentrations are not shown. Experiments were performed as biological duplicates; error bars represent the standard deviation. Kma K. marxianus, Wan W. anomalus, Eat1 unprocessed Eat1, trEat1 truncated Eat1