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Table 1 Strains used in this study

From: Multilevel optimisation of anaerobic ethyl acetate production in engineered Escherichia coli

Strain

Characteristics

Source

Escherichia coli BW25113 (DE3)

Wild type with integrated DE3 lysogen

[48]

Escherichia coli BW25113 ΔackAΔldhA

Disruption of lactate and acetate production (via ackA)

[20]

Escherichia coli T7 Express

fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS

λ DE3 = λ sBamHIo ∆EcoRI-B int::(LacI::PlacUV5::T7 gene1) i21 ∆nin5

NEB

Escherichia coli NEB® 5-alpha

fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17

NEB