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Table 1 Strains used in this study

From: Multilevel optimisation of anaerobic ethyl acetate production in engineered Escherichia coli

StrainCharacteristicsSource
Escherichia coli BW25113 (DE3)Wild type with integrated DE3 lysogen[48]
Escherichia coli BW25113 ΔackAΔldhADisruption of lactate and acetate production (via ackA)[20]
Escherichia coli T7 ExpressfhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS
λ DE3 = λ sBamHIo ∆EcoRI-B int::(LacI::PlacUV5::T7 gene1) i21 ∆nin5
NEB
Escherichia coli NEB® 5-alphafhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17NEB