Fig. 5
From: Mechanistic insights into pH-dependent H2 photoproduction in bisulfite-treated Chlamydomonas cells
Treatment with a combination of NaHSO3 and Na2SO3 significantly enhances the yield of H2 photoproduction in C. reinhardtii cells. a Effect of treatment with different concentrations of either NaHSO3 or Na2SO3 on PSII activity. The Chl concentration was adjusted to 10 µg mL−1 and under the intensity of 200 μE m−2 s−1, NaHSO3 or Na2SO3 with different concentrations was added to the cell cultures for 5 min before measurement. Subsequently, PSII activity was immediately measured using a Dual-PAM-100 monitoring system. b, c Effect of treatment with a combination of 7 mM NaHSO3 and 6 mM Na2SO3 on PSII activity (b) and H2 photoproduction (c) at different pH values. PSII activity was evaluated by calculated Fv/Fm values. Values are mean ± SD (n = 5). *p < 0.05; ***p < 0.001