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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Enzymatic conversion reactions of 5-hydroxymethylfurfural (HMF) to bio-based 2,5-diformylfuran (DFF) and 2,5-furandicarboxylic acid (FDCA) with air: mechanisms, pathways and synthesis selectivity

Fig. 2

Oxidation of different substrates with AO and FAD as a cofactor. Time course of oxidation of HMF (a), DFF (b) or FFA (c) by AO. Reaction conditions: final reaction volume 5 mL, 1 µM enzyme, 1 µM FAD, 10 mM HMF in 50 mM sodium phosphate buffer (pH 7) at 30 °C and constant stirring at 150 min−1. HMF 5-hydroxymethilfurfural, DFF 2,5-diformylfuran, FFA 5-formyl-2-furoic acid, FDCA 2,5-furandicarboxylic acid, AO alcohol oxidase from Pichia pastoris, FAD flavin adenine dinucleotide. The average relative error was ± 11% and was estimated based on selected repeated experiments

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