Fig. 3
The effect of FAD concentration on activity of AO against HMF. Oxidation of HMF with AO and different concentrations of FAD after 72 h. Reaction conditions: final reaction volume 5 mL, 1 µM enzyme, 1, 10 or 100 µM FAD, 10 mM HMF in 50 mM sodium phosphate buffer (pH 7) at 30 °C and constant stirring at 150 min−1. HMF 5-hydroxymethilfurfural, DFF 2,5-diformylfuran, FFA 5-formyl-2-furoic acid, AO alcohol oxidase from Pichia pastoris, FAD flavin adenine dinucleotide. The average relative error was ± 11% and was estimated based on selected repeated experiments