Table 3 Effect of substrate concentration on enzyme activity
Yield [%] | Substrate concentration [mM] | |||||
|---|---|---|---|---|---|---|
20 | 10 | 5 | 20 | 10 | 5 | |
AO + HMF | GAO + DFF | |||||
HMF | 76.5 | 67.2 | 57.1 | 2.3 | 2.2 | 2.1 |
DFF | 19.8 | 29.7 | 39.9 | 96.4 | 96.5 | 96.6 |
HMFA | – | – | – | – | – | – |
FFA | 3.7 | 3.1 | 3.0 | 1.3 | 1.3 | 1.3 |
FDCA | – | – | – | – | – | – |
LAC + HMFA | HRP + FFA | |||||
HMF | – | – | – | – | – | – |
DFF | – | – | – | – | – | – |
HMFA | 100 | 99.9 | 100 | – | – | – |
FFA | – | – | – | 99.1 | 99.4 | 99.3 |
FDCA | – | 0.1 | – | 0.9 | 0.6 | 0.7 |