Skip to main content

Table 3 Effect of substrate concentration on enzyme activity

From: Enzymatic conversion reactions of 5-hydroxymethylfurfural (HMF) to bio-based 2,5-diformylfuran (DFF) and 2,5-furandicarboxylic acid (FDCA) with air: mechanisms, pathways and synthesis selectivity

Yield [%]Substrate concentration [mM]
2010520105
 AO + HMFGAO + DFF
HMF76.567.257.12.32.22.1
DFF19.829.739.996.496.596.6
HMFA
FFA3.73.13.01.31.31.3
FDCA
 LAC + HMFAHRP + FFA
HMF
DFF
HMFA10099.9100
FFA99.199.499.3
FDCA0.10.90.60.7
  1. Oxidation of different concentrations of HMF, DFF, HMFA and FFA with different enzymes after 72 h. Reaction conditions: final reaction volume 5 mL, 1 or 8 µM enzyme (1 µM AO and 8 µM GAO, LAC and HRP), 5, 10 or 20 mM HMF, DFF, HMFA or FFA in 50 mM sodium phosphate buffer (pH 7) at 30 °C and constant stirring at 150 min−1. Reactions with AO also included 1 µM FAD. HMF 5-hydroxymethilfurfural, HMFA 5-hydroxymethyl-2-furoic acid, DFF 2,5-diformylfuran, FFA 5-formyl-2-furoic acid, AO alcohol oxidase from Pichia pastoris, GAO galactose oxidase from Dactylium dendroides, CAT catalase from Aspergillus niger, LAC laccase, HRP horseradish peroxidase. The average relative error was ± 11% and was estimated based on selected repeated experiments