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Table 1 Micrococcus luteus trpE16 strains used in this study

From: Distribution and diversity of olefins and olefin-biosynthesis genes in Gram-positive bacteria

StrainGenotype and relevant phenotypeSource
trpE16trpE16, Trp mutant of ATCC 27141Kloos and Rose [10]
trpE16 oleAex-MAW846M:kantrpE16 with exchange of native oleA gene (Mlut_13230) against heterologous oleA gene from Kocuria sp. MAW846M; KanrThis study
trpE16 oleABCDex-MAW846M:kantrpE16 with exchange of native oleABCD gene cluster (Mlut_13230) against heterologous oleABCD gene cluster from Kocuria sp. MAW846M; KanrThis study
opetrpE16 Pup13230 (replacement of native oleABCD gene cluster promoter by strong succinate dehydrogenase promoter)Surger et al. [16]
ope oleAex-2148:kanope with exchange of native oleA gene (Mlut_13230) against heterologous oleA gene from Micrococcus luteus 2148; KanrThis study
ope oleAex-20319:kanope with exchange of native oleA gene (Mlut_13230) against heterologous oleA gene from Ko. palustris DSM 20319; KanrThis study
ope oleAex-3352:kanope with exchange of native oleA gene (Mlut_13230) against heterologous oleA gene from Kocuria sp. 3352; KanrThis study
ope oleAex-TW93:kanope with exchange of native oleA gene (Mlut_13230) against heterologous oleA gene from Ky. sedentarius TW93; KanrThis study
  1. ope ole promoter exchange, Kanr kanamycin resistant