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Table 1 Micrococcus luteus trpE16 strains used in this study

From: Distribution and diversity of olefins and olefin-biosynthesis genes in Gram-positive bacteria

Strain

Genotype and relevant phenotype

Source

trpE16

trpE16, Trp− mutant of ATCC 27141

Kloos and Rose [10]

trpE16 oleAex-MAW846M:kan

trpE16 with exchange of native oleA gene (Mlut_13230) against heterologous oleA gene from Kocuria sp. MAW846M; Kanr

This study

trpE16 oleABCDex-MAW846M:kan

trpE16 with exchange of native oleABCD gene cluster (Mlut_13230) against heterologous oleABCD gene cluster from Kocuria sp. MAW846M; Kanr

This study

ope

trpE16 Pup13230 (replacement of native oleABCD gene cluster promoter by strong succinate dehydrogenase promoter)

Surger et al. [16]

ope oleAex-2148:kan

ope with exchange of native oleA gene (Mlut_13230) against heterologous oleA gene from Micrococcus luteus 2148; Kanr

This study

ope oleAex-20319:kan

ope with exchange of native oleA gene (Mlut_13230) against heterologous oleA gene from Ko. palustris DSM 20319; Kanr

This study

ope oleAex-3352:kan

ope with exchange of native oleA gene (Mlut_13230) against heterologous oleA gene from Kocuria sp. 3352; Kanr

This study

ope oleAex-TW93:kan

ope with exchange of native oleA gene (Mlut_13230) against heterologous oleA gene from Ky. sedentarius TW93; Kanr

This study

  1. ope ole promoter exchange, Kanr kanamycin resistant