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Table 1 Melting temperatures for various CkXyn10C-GE15A domains, as determined using a Thermofluor assay

From: Investigation of a thermostable multi-domain xylanase-glucuronoyl esterase enzyme from Caldicellulosiruptor kristjanssonii incorporating multiple carbohydrate-binding modules

Domain

Temperature (°C)

CBM22.2

62.6 ± 1.8

CkXyn10C

ND

CBM22-CBM22-CkXyn10C

78.6 ± 0.1

CBM9.1

ND

CBM9.2

49.1 ± 3.1

CBM9.3

42.6 ± 1.8

CkGE15A

72.0 ± 0.2

  1. The results are presented as the average of triplicate experiments with standard errors
  2. ND unable to be determined using this assay
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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