Skip to main content

Table 3 Kinetic parameters for CkXyn10C, assays run using 50 nM GH10 for 5 min

From: Investigation of a thermostable multi-domain xylanase-glucuronoyl esterase enzyme from Caldicellulosiruptor kristjanssonii incorporating multiple carbohydrate-binding modules

Condition

pH 7

pH 5.5

Km (g/L)

kcat (1/s)

Km (g/L)

kcat (1/s)

Birchwood xylan, 40 °C

ND

ND

N/A

N/A

Birchwood xylan, 65 °C

6.2 ± 0.8

80 ± 4.8

5.9 ± 1.1

173 ± 14.4

Beechwood xylan, 40 °C

14.6 ± 4.8

110 ± 22.1

N/A

N/A

Beechwood xylan, 65 °C

5.1 ± 0.7

173 ± 10.7

2.6 ± 0.3

387 ± 13.6

Wheat arabinoxylan, 65 °C

56.3 ± 21.7

1940 ± 627

10.0 ± 1.4

960 ± 74.2

  1. The results are presented as the average of triplicate experiments with standard errors
  2. ND not determined due to low activity, N/A not assayed
Back to article page

Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

Contact us