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Table 3 Kinetic parameters for CkXyn10C, assays run using 50 nM GH10 for 5 min

From: Investigation of a thermostable multi-domain xylanase-glucuronoyl esterase enzyme from Caldicellulosiruptor kristjanssonii incorporating multiple carbohydrate-binding modules

ConditionpH 7pH 5.5
Km (g/L)kcat (1/s)Km (g/L)kcat (1/s)
Birchwood xylan, 40 °CNDNDN/AN/A
Birchwood xylan, 65 °C6.2 ± 0.880 ± 4.85.9 ± 1.1173 ± 14.4
Beechwood xylan, 40 °C14.6 ± 4.8110 ± 22.1N/AN/A
Beechwood xylan, 65 °C5.1 ± 0.7173 ± 10.72.6 ± 0.3387 ± 13.6
Wheat arabinoxylan, 65 °C56.3 ± 21.71940 ± 62710.0 ± 1.4960 ± 74.2
  1. The results are presented as the average of triplicate experiments with standard errors
  2. ND not determined due to low activity, N/A not assayed