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Table 4 Kinetic parameters for CBM22-CBM22-CkXyn10C, assays run using 500 nM CBM-CBM22-CkXyn10C for 5 min

From: Investigation of a thermostable multi-domain xylanase-glucuronoyl esterase enzyme from Caldicellulosiruptor kristjanssonii incorporating multiple carbohydrate-binding modules

ConditionpH 7pH 5.5
Km (g/L)kcat (1/s)Km (g/L)kcat (1/s)
Birchwood xylan, 40 °C20.4 ± 2.934 ± 3.2N/AN/A
Birchwood xylan, 65 °C9.6 ± 0.581 ± 2.4N/AN/A
Birchwood xylan, 80 °C6.6 ± 0.480 ± 210.2 ± 1366 ± 24
Beechwood xylan, 40 °C11.5 ± 1.523 ± 1.7N/AN/A
Beechwood xylan, 65 °C9.6 ± 0.677 ± 2.7N/AN/A
Beechwood xylan, 80 °C10.5 ± 0.7110 ± 4.28.4 ± 1.4321 ± 17
Wheat arabinoxylan, 80 °C
(50 nM enzyme)
8.4 ± 1.2633 ± 4.513.2 ± 21953 ± 18
  1. The results are presented as the average of triplicate experiments with standard errors
  2. N/A not assayed