Skip to main content

Table 4 Kinetic parameters for CBM22-CBM22-CkXyn10C, assays run using 500 nM CBM-CBM22-CkXyn10C for 5 min

From: Investigation of a thermostable multi-domain xylanase-glucuronoyl esterase enzyme from Caldicellulosiruptor kristjanssonii incorporating multiple carbohydrate-binding modules

Condition

pH 7

pH 5.5

Km (g/L)

kcat (1/s)

Km (g/L)

kcat (1/s)

Birchwood xylan, 40 °C

20.4 ± 2.9

34 ± 3.2

N/A

N/A

Birchwood xylan, 65 °C

9.6 ± 0.5

81 ± 2.4

N/A

N/A

Birchwood xylan, 80 °C

6.6 ± 0.4

80 ± 2

10.2 ± 1

366 ± 24

Beechwood xylan, 40 °C

11.5 ± 1.5

23 ± 1.7

N/A

N/A

Beechwood xylan, 65 °C

9.6 ± 0.6

77 ± 2.7

N/A

N/A

Beechwood xylan, 80 °C

10.5 ± 0.7

110 ± 4.2

8.4 ± 1.4

321 ± 17

Wheat arabinoxylan, 80 °C

(50 nM enzyme)

8.4 ± 1.2

633 ± 4.5

13.2 ± 2

1953 ± 18

  1. The results are presented as the average of triplicate experiments with standard errors
  2. N/A not assayed