Fig. 2

Improved ethyl acetate production by N-terminal truncated versions of Kma Eat1. a, b Ethyl acetate titres reached by cultures producing Kma trEat1 variants at 0.01 mM IPTG after a 24 h and b 144 h. c, d Ethyl acetate titres reached by cultures producing Kma trEat1 variants at 0.1 mM IPTG after c 24 h and d 126 h. Strains were grown under anaerobic conditions in modified M9 medium. Genes were expressed from a series of pET26b plasmids. Experiments were performed as biological duplicates; error bars represent the standard deviation. Abbreviations: Kma, K. marxianus