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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: From Eat to trEat: engineering the mitochondrial Eat1 enzyme for enhanced ethyl acetate production in Escherichia coli

Fig. 4

Improved ethyl acetate production by N-terminal truncated versions of Wan Eat1. a truncated variants of the Wan Eat1 N-terminus based on the Kma trEat1 Q-28 and K-30. The highlighted LP residues indicate the first region that is conserved in all known Eat1 proteins. b Ethyl acetate titres reached by cultures producing Wan trEat1 variants at 0.01 mM IPTG induction levels after 24 h (dark blue) and 120 h (light blue). c Ethyl acetate titres reached by cultures producing Wan trEat1 variants at 0.001 mM IPTG induction levels after 24 h (dark blue) and 120 h (light blue) Strains were grown under anaerobic conditions in modified M9 medium. Genes were expressed from a series of pET26b plasmids. Experiments were performed as biological duplicates; error bars represent the standard deviation. Wan—W. anomalus, upEat1—unprocessed Eat1

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