Table 2 Plasmids used in this study

pET26b

LacI/T7

/

This study

pET26b:hWanEat1

LacI/T7

Codon harmonised eat1 from Wickerhamomyces anomalus DSM 6766

[7]

pET26b:hKmaEat1

LacI/T7

Codon harmonised eat1 from Kluyveromyces marxianus DSM 5422

This study

pET26b:hKma trEat1A-4

LacI/T7

Kma Eat1 truncated at A-4

This study

pET26b:hKma trEat1 P-9

LacI/T7

Kma Eat1 truncated at P-9

This study

pET26b:hKma trEat1 T-15

LacI/T7

Kma Eat1 truncated at T-15

This study

pET26b:hKma trEat1 Y-19

LacI/T7

Kma Eat1 truncated at Y-19

This study

pET26b:hKma trEat1 S-20

LacI/T7

Kma Eat1 truncated at S-20

This study

pET26b:hKma trEat1 R-24

LacI/T7

Kma Eat1 truncated at R-24

This study

pET26b:hKma trEat1 F-26

LacI/T7

Kma Eat1 truncated at F-26

This study

pET26b:hKma trEat1 N-27

LacI/T7

Kma Eat1 truncated at N-27

This study

pET26b:hKma trEat1 Q-28

LacI/T7

Kma Eat1 truncated at Q-28

This study

pET26b:hKma-trEat1-K30

LacI/T7

Kma Eat1 truncated at K-30

This study

pET26b:hKma-trEat1-P34

LacI/T7

Kma Eat1 truncated at P-34

This study

pET26b:hKma-trEat1-L35

LacI/T7

Kma Eat1 truncated at L-35

This study

pET26b:hKma-trEat1-P36

LacI/T7

Kma Eat1 truncated at P-36

This study

pET26b:hKma-trEat1-I37

LacI/T7

Kma Eat1 truncated at I-37

This study

pET2b:hWan-trEat1-V11

LacI/T7

Wan Eat1 truncated at V-11

This study

pET26b:hWan-trEat1-N13

LacI/T7

Wan Eat1 truncated at N-13

This study

pCas9

/

[6]

pTarget

/

[6]

pTarget-ackA

/

This study

pTarget-ldhA

/

This study