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Table 1 Quantitative 13C-IS py-GC–MS structural characterization of untreated and 7 days P. anserina-treated wheat straw lignin; corrected for relative response factors and relative abundance of 13C analogues

From: Evidence for ligninolytic activity of the ascomycete fungus Podospora anserina

 Control7 days
Lignin subunits (%)
 H8.9 ± 0.18.6 ± 0.0**
 G60.9 ± 0.559.3 ± 0.2**
 S30.2 ± 0.532.1 ± 0.2**
  S/G0.50 ± 0.00.54 ± 0.0**
Structural moieties (%)
 Unsubstituted6.4 ± 0.36.6 ± 0.1
 Methyl3.0 ± 0.23.3 ± 0.0*
 Vinyl32.4 ± 1.130.4 ± 0.3*
  4-VPa7.2 ± 0.16.6 ± 0.0**
  4-VGb21.7 ± 1.119.9 ± 0.3
 Cα-ox4.0 ± 0.14.3 ± 0.1*
  Diketones0.16 ± 0.00.25 ± 0.0**
 Cβ-oxc2.5 ± 0.12.5 ± 0.0
 Cγ-ox48.2 ± 1.149.1 ± 0.4
 Miscellaneous3.6 ± 0.13.8 ± 0.0
 PhCdγ52.6 ± 1.153.9 ± 0.4
 PhCγ-diketonese52.4 ± 1.153.6 ± 0.4
  1. Sum based on structural classification according to van Erven et al. [18, 19]. Average and standard deviation of analytical duplicates of biological triplicates. Structural features for the combined fractions weighted on the basis of the lignin mass balance
  2. a4-Vinylphenol. b4-Vinylguaiacol. cExcluding diketones. dPhenols with intact α, β, γ carbon side chain. ePhenols with intact α, β, γ carbon side chain, excluding diketones
  3. Significant differences calculated by two-tailed t-test (*p < 0.05, **p < 0.01)