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Table 1 Quantitative 13C-IS py-GC–MS structural characterization of untreated and 7 days P. anserina-treated wheat straw lignin; corrected for relative response factors and relative abundance of 13C analogues

From: Evidence for ligninolytic activity of the ascomycete fungus Podospora anserina

 

Control

7 days

Lignin subunits (%)

 H

8.9 ± 0.1

8.6 ± 0.0**

 G

60.9 ± 0.5

59.3 ± 0.2**

 S

30.2 ± 0.5

32.1 ± 0.2**

  S/G

0.50 ± 0.0

0.54 ± 0.0**

Structural moieties (%)

 Unsubstituted

6.4 ± 0.3

6.6 ± 0.1

 Methyl

3.0 ± 0.2

3.3 ± 0.0*

 Vinyl

32.4 ± 1.1

30.4 ± 0.3*

  4-VPa

7.2 ± 0.1

6.6 ± 0.0**

  4-VGb

21.7 ± 1.1

19.9 ± 0.3

 Cα-ox

4.0 ± 0.1

4.3 ± 0.1*

  Diketones

0.16 ± 0.0

0.25 ± 0.0**

 Cβ-oxc

2.5 ± 0.1

2.5 ± 0.0

 Cγ-ox

48.2 ± 1.1

49.1 ± 0.4

 Miscellaneous

3.6 ± 0.1

3.8 ± 0.0

 PhC dγ

52.6 ± 1.1

53.9 ± 0.4

 PhCγ-diketonese

52.4 ± 1.1

53.6 ± 0.4

  1. Sum based on structural classification according to van Erven et al. [18, 19]. Average and standard deviation of analytical duplicates of biological triplicates. Structural features for the combined fractions weighted on the basis of the lignin mass balance
  2. a4-Vinylphenol. b4-Vinylguaiacol. cExcluding diketones. dPhenols with intact α, β, γ carbon side chain. ePhenols with intact α, β, γ carbon side chain, excluding diketones
  3. Significant differences calculated by two-tailed t-test (*p < 0.05, **p < 0.01)