Table 1 Plasmids and strains used in this work
Plasmids/strains | Description | References |
|---|---|---|
Plasmids | ||
 pACYCDuet-1 | p15A origin; CmR; PT7 | Novagen |
 pHB5 | p15A origin; CmR; PT7::GPPS2-SabS1 | [6] |
 pHB7 | P15A origin; CmR; PT7::mvaE-mvaS-GPPS2-SabS1 | [6] |
 pTrcLower | ColE1 origin; AmpR; Ptrc::ERG12-ERG8-ERG19-IDI1 | [6] |
 pACYC-ybcK | p15A origin; CmR; PT7::ybcK | This work |
 pACYC-scpA | p15A origin; CmR; PT7::scpA | This work |
 pACYC-ygiZ | p15A origin; CmR; PT7::ygiZ | This work |
 pACYC-ybcK-GP PS2-SabS1 | p15A origin; CmR; PT7::ybcK-GPPS2-SabS1 | This work |
 pACYC-scpA-GP PS2-SabS1 | p15A origin; CmR; PT7::scpA-GPPS2-SabS1 | This work |
 pACYC-ygiZ-GPPS2-SabS1 | p15A origin; CmR; PT7::ygiZ-GPPS2-SabS1 | This work |
 pET28a-mvaE-mvaS | pBR322 origin; KanR; PT7:: mvaE-mvaS | This work |
 pGRB | pBR322 origin; AmpR; J23119 | [63] |
 pREDCas9 | pSC101 origin; SpcR; PlacUV5 | [63] |
Strains | ||
 DH5α | deoR, recA1, endA1, hsdR17(rk−, mk+), phoA, supE44, λ-, thi−1, gyrA96, relA1 | Invitrogen |
 BL21(DE3) | E. coli B dcm ompT hsdS(rB− mB−) gal | Invitrogen |
 XYF(DE3) | Strain after ALE | This work |
 ΔybcK | BL21(DE3) ybck knock-out strain | This work |
 XYFHB7 | XYF(DE3) harboring pHB7 and pTrcLower | This work |
 HB4 | BL21(DE3) harboring pHB7 and pTrcLower | [6] |
 WUT1 | BL21(DE3) harboring pACYCDuet-1 | This work |
 WUT2 | BL21(DE3) harboring pACYC-ybcK | This work |
 WUT3 | BL21(DE3) harboring pACYC-scpA | This work |
 WUT4 | BL21(DE3) harboring pACYC-ygiZ | This work |
 WUT14 | ΔybcK harboring pACYCDuet-1 | This work |
 WUT15 | BL21(DE3) harboring pHB5, pTrcLower and pET28a-mvaE-mvaS | This work |
 WUT16 | BL21(DE3) harboring pACYC-ybcK-GPPS2-SabS1, pTrcLower and pET28a-mvaE-mvaS | This work |
 WUT17 | BL21(DE3) harboring pACYC-scpA-GPPS2-SabS1, pTrcLower and pET28a-mvaE-mvaS | This work |
 WUT18 | BL21(DE3) harboring pACYC-ygiZ-GPPS2-SabS1, pTrcLower and pET28a-mvaE-mvaS | This work |