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Table 1 Plasmids and strains used in this work

From: Improvement of sabinene tolerance of Escherichia coli using adaptive laboratory evolution and omics technologies

Plasmids/strains Description References
Plasmids
 pACYCDuet-1 p15A origin; CmR; PT7 Novagen
 pHB5 p15A origin; CmR; PT7::GPPS2-SabS1 [6]
 pHB7 P15A origin; CmR; PT7::mvaE-mvaS-GPPS2-SabS1 [6]
 pTrcLower ColE1 origin; AmpR; Ptrc::ERG12-ERG8-ERG19-IDI1 [6]
 pACYC-ybcK p15A origin; CmR; PT7::ybcK This work
 pACYC-scpA p15A origin; CmR; PT7::scpA This work
 pACYC-ygiZ p15A origin; CmR; PT7::ygiZ This work
 pACYC-ybcK-GP PS2-SabS1 p15A origin; CmR; PT7::ybcK-GPPS2-SabS1 This work
 pACYC-scpA-GP PS2-SabS1 p15A origin; CmR; PT7::scpA-GPPS2-SabS1 This work
 pACYC-ygiZ-GPPS2-SabS1 p15A origin; CmR; PT7::ygiZ-GPPS2-SabS1 This work
 pET28a-mvaE-mvaS pBR322 origin; KanR; PT7:: mvaE-mvaS This work
 pGRB pBR322 origin; AmpR; J23119 [63]
 pREDCas9 pSC101 origin; SpcR; PlacUV5 [63]
Strains
 DH5α deoR, recA1, endA1, hsdR17(rk, mk+), phoA, supE44, λ-, thi1, gyrA96, relA1 Invitrogen
 BL21(DE3) E. coli B dcm ompT hsdS(rB mB) gal Invitrogen
 XYF(DE3) Strain after ALE This work
 ΔybcK BL21(DE3) ybck knock-out strain This work
 XYFHB7 XYF(DE3) harboring pHB7 and pTrcLower This work
 HB4 BL21(DE3) harboring pHB7 and pTrcLower [6]
 WUT1 BL21(DE3) harboring pACYCDuet-1 This work
 WUT2 BL21(DE3) harboring pACYC-ybcK This work
 WUT3 BL21(DE3) harboring pACYC-scpA This work
 WUT4 BL21(DE3) harboring pACYC-ygiZ This work
 WUT14 ΔybcK harboring pACYCDuet-1 This work
 WUT15 BL21(DE3) harboring pHB5, pTrcLower and pET28a-mvaE-mvaS This work
 WUT16 BL21(DE3) harboring pACYC-ybcK-GPPS2-SabS1, pTrcLower and pET28a-mvaE-mvaS This work
 WUT17 BL21(DE3) harboring pACYC-scpA-GPPS2-SabS1, pTrcLower and pET28a-mvaE-mvaS This work
 WUT18 BL21(DE3) harboring pACYC-ygiZ-GPPS2-SabS1, pTrcLower and pET28a-mvaE-mvaS This work