Skip to main content

Table 1 Plasmids and strains used in this work

From: Improvement of sabinene tolerance of Escherichia coli using adaptive laboratory evolution and omics technologies

Plasmids/strainsDescriptionReferences
Plasmids
 pACYCDuet-1p15A origin; CmR; PT7Novagen
 pHB5p15A origin; CmR; PT7::GPPS2-SabS1[6]
 pHB7P15A origin; CmR; PT7::mvaE-mvaS-GPPS2-SabS1[6]
 pTrcLowerColE1 origin; AmpR; Ptrc::ERG12-ERG8-ERG19-IDI1[6]
 pACYC-ybcKp15A origin; CmR; PT7::ybcKThis work
 pACYC-scpAp15A origin; CmR; PT7::scpAThis work
 pACYC-ygiZp15A origin; CmR; PT7::ygiZThis work
 pACYC-ybcK-GP PS2-SabS1p15A origin; CmR; PT7::ybcK-GPPS2-SabS1This work
 pACYC-scpA-GP PS2-SabS1p15A origin; CmR; PT7::scpA-GPPS2-SabS1This work
 pACYC-ygiZ-GPPS2-SabS1p15A origin; CmR; PT7::ygiZ-GPPS2-SabS1This work
 pET28a-mvaE-mvaSpBR322 origin; KanR; PT7:: mvaE-mvaSThis work
 pGRBpBR322 origin; AmpR; J23119[63]
 pREDCas9pSC101 origin; SpcR; PlacUV5[63]
Strains
 DH5αdeoR, recA1, endA1, hsdR17(rk−, mk+), phoA, supE44, λ-, thi−1, gyrA96, relA1Invitrogen
 BL21(DE3)E. coli B dcm ompT hsdS(rB− mB−) galInvitrogen
 XYF(DE3)Strain after ALEThis work
 ΔybcKBL21(DE3) ybck knock-out strainThis work
 XYFHB7XYF(DE3) harboring pHB7 and pTrcLowerThis work
 HB4BL21(DE3) harboring pHB7 and pTrcLower[6]
 WUT1BL21(DE3) harboring pACYCDuet-1This work
 WUT2BL21(DE3) harboring pACYC-ybcKThis work
 WUT3BL21(DE3) harboring pACYC-scpAThis work
 WUT4BL21(DE3) harboring pACYC-ygiZThis work
 WUT14ΔybcK harboring pACYCDuet-1This work
 WUT15BL21(DE3) harboring pHB5, pTrcLower and pET28a-mvaE-mvaSThis work
 WUT16BL21(DE3) harboring pACYC-ybcK-GPPS2-SabS1, pTrcLower and pET28a-mvaE-mvaSThis work
 WUT17BL21(DE3) harboring pACYC-scpA-GPPS2-SabS1, pTrcLower and pET28a-mvaE-mvaSThis work
 WUT18BL21(DE3) harboring pACYC-ygiZ-GPPS2-SabS1, pTrcLower and pET28a-mvaE-mvaSThis work
Back to article page