|
Plasmids
|
|
pACYCDuet-1
|
p15A origin; CmR; PT7
|
Novagen
|
|
pHB5
|
p15A origin; CmR; PT7::GPPS2-SabS1
|
[6]
|
|
pHB7
|
P15A origin; CmR; PT7::mvaE-mvaS-GPPS2-SabS1
|
[6]
|
|
pTrcLower
|
ColE1 origin; AmpR; Ptrc::ERG12-ERG8-ERG19-IDI1
|
[6]
|
|
pACYC-ybcK
|
p15A origin; CmR; PT7::ybcK
|
This work
|
|
pACYC-scpA
|
p15A origin; CmR; PT7::scpA
|
This work
|
|
pACYC-ygiZ
|
p15A origin; CmR; PT7::ygiZ
|
This work
|
|
pACYC-ybcK-GP PS2-SabS1
|
p15A origin; CmR; PT7::ybcK-GPPS2-SabS1
|
This work
|
|
pACYC-scpA-GP PS2-SabS1
|
p15A origin; CmR; PT7::scpA-GPPS2-SabS1
|
This work
|
|
pACYC-ygiZ-GPPS2-SabS1
|
p15A origin; CmR; PT7::ygiZ-GPPS2-SabS1
|
This work
|
|
pET28a-mvaE-mvaS
|
pBR322 origin; KanR; PT7:: mvaE-mvaS
|
This work
|
|
pGRB
|
pBR322 origin; AmpR; J23119
|
[63]
|
|
pREDCas9
|
pSC101 origin; SpcR; PlacUV5
|
[63]
|
|
Strains
|
|
DH5α
|
deoR, recA1, endA1, hsdR17(rk−, mk+), phoA, supE44, λ-, thi−1, gyrA96, relA1
|
Invitrogen
|
|
BL21(DE3)
|
E. coli B dcm ompT hsdS(rB− mB−) gal
|
Invitrogen
|
|
XYF(DE3)
|
Strain after ALE
|
This work
|
|
ΔybcK
|
BL21(DE3) ybck knock-out strain
|
This work
|
|
XYFHB7
|
XYF(DE3) harboring pHB7 and pTrcLower
|
This work
|
|
HB4
|
BL21(DE3) harboring pHB7 and pTrcLower
|
[6]
|
|
WUT1
|
BL21(DE3) harboring pACYCDuet-1
|
This work
|
|
WUT2
|
BL21(DE3) harboring pACYC-ybcK
|
This work
|
|
WUT3
|
BL21(DE3) harboring pACYC-scpA
|
This work
|
|
WUT4
|
BL21(DE3) harboring pACYC-ygiZ
|
This work
|
|
WUT14
|
ΔybcK harboring pACYCDuet-1
|
This work
|
|
WUT15
|
BL21(DE3) harboring pHB5, pTrcLower and pET28a-mvaE-mvaS
|
This work
|
|
WUT16
|
BL21(DE3) harboring pACYC-ybcK-GPPS2-SabS1, pTrcLower and pET28a-mvaE-mvaS
|
This work
|
|
WUT17
|
BL21(DE3) harboring pACYC-scpA-GPPS2-SabS1, pTrcLower and pET28a-mvaE-mvaS
|
This work
|
|
WUT18
|
BL21(DE3) harboring pACYC-ygiZ-GPPS2-SabS1, pTrcLower and pET28a-mvaE-mvaS
|
This work
|
