Fig. 2

Map of the expression plasmid (pDBV), and fatty acid contents of constructs in Leptolyngbya BL0902. a To generate the pDBV (and other) plasmids, pAM4418-derived expression vectors were generated with synthetic genes designed to express: (i) the Δ6 desaturase (DesD) from Synechocystis sp. PCC 6803, (ii) the “Δ15” (ω3, or methyl-end) desaturase (DesB) from Synechococcus sp. PCC 7002, and/or (iii) the “vesicle-inducing protein in plastids” (Vipp1) from Synechococcus sp. PCC 7002. Included in the plasmid vector are aadA, conferring resistance to spectinomycin and streptomycin, as well as trpA and rrnB which block continued transcription. b Plasmids with one, two or all three of the inserted cyanobacterial genes were constructed and conjugated into Leptolyngbya sp. strain BL0902, transconjugants were selected on BG-11 agar plates containing spectinomycin and streptomycin, and cultures were grown at 30 °C in BG-11 media, harvested and dried for fatty acid analysis of lipid content by fatty acid methyl ester (FAME) analysis (gas chromatography with flame ionization detection, GC-FID). Shown are total saturated (magenta), monounsaturated (dark blue) and polyunsaturated (cyan) fatty acid contents (n = 3 or more, from left to right, except n = 2 for pDV)