Fig. 3

Quantitative analysis showing 18- and 20-carbon polyunsaturated fatty acids in wild type and engineered cyanobacteria. a, b PUFA analyses of plasmid-bearing Leptolyngbya sp. strain BL0902 as described in Fig. 2b are shown as mean ± standard deviation for wild type (WT) and single-gene constructs (a), or double and triple-gene constructs (b), expressed as the mol percent of total fatty acids (very similar to weight percent values). (C) PUFAs produced by WT and pDBV-bearing species of cyanobacteria, including Leptolyngbya sp. strain BL0902, Synechococcus sp. PCC 7002 and Anabaena sp. PCC 7120, are shown as averages of the mass (mg per g of dry weight) ± standard deviation (n = 3 or more, from left to right, except n = 2 and 1 for the 7120 samples). a–c Those exhibiting a statistically significant difference in content compared with WT by a Student’s t test are indicated with asterisks (*p < 0.05, **p < 0.01, ***p < 0.001)