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Table 3 Content of 18C omega-3 fatty acids in engineered cyanobacteria from this and previous studies

From: Acyl-lipid desaturases and Vipp1 cooperate in cyanobacteria to produce novel omega-3 PUFA-containing glycolipids

SpeciesVector for engineeringTemp (°C)Photon flux density, µmol photons (m2· s)−1ALA (18:3, n-3), mol% of total FAsaSDA (18:4, n-3), mol% of total FAsaReferences
Leptolyngbya sp. strain BL0902None (wt)3020–3022.6 ± 11.5n.d.This study
With pBV (to express DesB and Vipp1)b3020–3037.7 ± 1.7n.d.
With pDBV (to express DesD, DesB and Vipp1)b3020–309.3 ± 0.926.6 ± 1.0
Synechococcus elongatus PCC 7942None (wt)2260n.d.n.r.Santos-Merino et al. [54]
MSM26 (strain with overexpressed desA and desB)c22607.6 ± 1.5n.r.
222508.8 ± 1.8n.r.
MSM45 (strain with ΔfabD (deleted), and overexpressed fabF, desA and desB)c226022.6 ± 1.5n.r.
Synechococcus sp. strain NKBG 15041cEmpty vector2550~21 ± 4dn.d.Yoshino et al. [47]
Vector for desD overexpressione255020.6 ± 3.72.1 ± 0.5
2510045.7 ± 7.45.1 ± 0.6
2515053.1 ± 1.36.2 ± 0.5
30509.0 ± 6.30.9 ± 0.4
Synechococcus sp. PCC7002None (wt)30605.2 ± 0.3n.d.Dong et al. [80]
Syd15D (for desB overexpression)f306028.3 ± 2.3n.d.
Syd6Dd15D (for desD and desB overexpression)e,f30606.6 ± 0.711.6 ± 1.6
Synechocystis sp. PCC 6803None (wt)20402.5 ± 0.31.5 ± 0.2Chen et al. [46]
30401.5 ± 0.21.2 ± 0.3
Sy15 (for desB overexpression)g204023.1 ± 2.310.8 ± 1.6
304017.5 ± 2.39.1 ± 1.3
Sy15Sy6 (for desB and desD overexpression)g,h204016.4 ± 1.913.1 ± 1.3
304023.6 ± 3.47.8 ± 0.7
  1. n.d. not detected, n.r. not reported
  2. aExpressed as mean ± standard deviation
  3. bpBV encodes Synechococcus sp. PCC7002 DesB for Δ15 desaturase (also known as ω3 or methyl end desaturase) and Synechococcus sp. PCC7002 Vipp1 for inducing thylakoid membranes; pDBV is the three-gene plasmid derived from pAM4418 (Fig. 2) developed in this work which includes the two genes of pBV plus Synechocystis sp. PCC6803 desD for Δ6 desaturase expression
  4. cdesA and desB were from Synechococcus sp. PCC7002
  5. dEstimated from mass if total fatty acid content is same as transformed strain
  6. edesD was from Synechocystis sp. PCC6803
  7. fdesB was from Synechocystis sp. PCC6803
  8. gdesB was from Synechocystis sp. PCC6803 plus a C-terminal FLAG tag
  9. hdesD was from Synechocystis sp. PCC6803 plus a C-terminal His tag