Species | Vector for engineering | Temp (°C) | Photon flux density, µmol photons (m2· s)−1 | ALA (18:3, n-3), mol% of total FAsa | SDA (18:4, n-3), mol% of total FAsa | References |
---|---|---|---|---|---|---|
Leptolyngbya sp. strain BL0902 | None (wt) | 30 | 20–30 | 22.6 ± 11.5 | n.d. | This study |
With pBV (to express DesB and Vipp1)b | 30 | 20–30 | 37.7 ± 1.7 | n.d. | ||
With pDBV (to express DesD, DesB and Vipp1)b | 30 | 20–30 | 9.3 ± 0.9 | 26.6 ± 1.0 | ||
Synechococcus elongatus PCC 7942 | None (wt) | 22 | 60 | n.d. | n.r. | Santos-Merino et al. [54] |
MSM26 (strain with overexpressed desA and desB)c | 22 | 60 | 7.6 ± 1.5 | n.r. | ||
22 | 250 | 8.8 ± 1.8 | n.r. | |||
MSM45 (strain with ΔfabD (deleted), and overexpressed fabF, desA and desB)c | 22 | 60 | 22.6 ± 1.5 | n.r. | ||
Synechococcus sp. strain NKBG 15041c | Empty vector | 25 | 50 | ~21 ± 4d | n.d. | Yoshino et al. [47] |
Vector for desD overexpressione | 25 | 50 | 20.6 ± 3.7 | 2.1 ± 0.5 | ||
25 | 100 | 45.7 ± 7.4 | 5.1 ± 0.6 | |||
25 | 150 | 53.1 ± 1.3 | 6.2 ± 0.5 | |||
30 | 50 | 9.0 ± 6.3 | 0.9 ± 0.4 | |||
Synechococcus sp. PCC7002 | None (wt) | 30 | 60 | 5.2 ± 0.3 | n.d. | Dong et al. [80] |
Syd15D (for desB overexpression)f | 30 | 60 | 28.3 ± 2.3 | n.d. | ||
Syd6Dd15D (for desD and desB overexpression)e,f | 30 | 60 | 6.6 ± 0.7 | 11.6 ± 1.6 | ||
Synechocystis sp. PCC 6803 | None (wt) | 20 | 40 | 2.5 ± 0.3 | 1.5 ± 0.2 | Chen et al. [46] |
30 | 40 | 1.5 ± 0.2 | 1.2 ± 0.3 | |||
Sy15 (for desB overexpression)g | 20 | 40 | 23.1 ± 2.3 | 10.8 ± 1.6 | ||
30 | 40 | 17.5 ± 2.3 | 9.1 ± 1.3 | |||
Sy15Sy6 (for desB and desD overexpression)g,h | 20 | 40 | 16.4 ± 1.9 | 13.1 ± 1.3 | ||
30 | 40 | 23.6 ± 3.4 | 7.8 ± 0.7 |