Acyl-lipid desaturases and Vipp1 cooperate in cyanobacteria to produce novel omega-3 PUFA-containing glycolipids

Poole, Leslie B.; Parsonage, Derek; Sergeant, Susan; Miller, Leslie R.; Lee, Jingyun; Furdui, Cristina M.; Chilton, Floyd H.

doi:10.1186/s13068-020-01719-7

Biotechnology for Biofuels and Bioproducts

Table 3 Content of 18C omega-3 fatty acids in engineered cyanobacteria from this and previous studies

From: Acyl-lipid desaturases and Vipp1 cooperate in cyanobacteria to produce novel omega-3 PUFA-containing glycolipids

Species	Vector for engineering	Temp (°C)	Photon flux density, µmol photons (m²· s)⁻¹	ALA (18:3, n-3), mol% of total FAs^a	SDA (18:4, n-3), mol% of total FAs^a	References
Leptolyngbya sp. strain BL0902	None (wt)	30	20–30	22.6 ± 11.5	n.d.	This study
	With pBV (to express DesB and Vipp1)^b	30	20–30	37.7 ± 1.7	n.d.
	With pDBV (to express DesD, DesB and Vipp1)^b	30	20–30	9.3 ± 0.9	26.6 ± 1.0
Synechococcus elongatus PCC 7942	None (wt)	22	60	n.d.	n.r.	Santos-Merino et al. [54]
	MSM26 (strain with overexpressed desA and desB)^c	22	60	7.6 ± 1.5	n.r.
	MSM26 (strain with overexpressed desA and desB)^c	22	250	8.8 ± 1.8	n.r.
	MSM45 (strain with ΔfabD (deleted), and overexpressed fabF, desA and desB)^c	22	60	22.6 ± 1.5	n.r.
Synechococcus sp. strain NKBG 15041c	Empty vector	25	50	~21 ± 4^d	n.d.	Yoshino et al. [47]
	Vector for desD overexpression^e	25	50	20.6 ± 3.7	2.1 ± 0.5
		25	100	45.7 ± 7.4	5.1 ± 0.6
		25	150	53.1 ± 1.3	6.2 ± 0.5
		30	50	9.0 ± 6.3	0.9 ± 0.4
Synechococcus sp. PCC7002	None (wt)	30	60	5.2 ± 0.3	n.d.	Dong et al. [80]
	Syd15D (for desB overexpression)^f	30	60	28.3 ± 2.3	n.d.
	Syd6Dd15D (for desD and desB overexpression)^e,f	30	60	6.6 ± 0.7	11.6 ± 1.6
Synechocystis sp. PCC 6803	None (wt)	20	40	2.5 ± 0.3	1.5 ± 0.2	Chen et al. [46]
	None (wt)	30	40	1.5 ± 0.2	1.2 ± 0.3
	Sy15 (for desB overexpression)^g	20	40	23.1 ± 2.3	10.8 ± 1.6
	Sy15 (for desB overexpression)^g	30	40	17.5 ± 2.3	9.1 ± 1.3
	Sy15Sy6 (for desB and desD overexpression)^g,h	20	40	16.4 ± 1.9	13.1 ± 1.3
	Sy15Sy6 (for desB and desD overexpression)^g,h	30	40	23.6 ± 3.4	7.8 ± 0.7

n.d. not detected, n.r. not reported
^aExpressed as mean ± standard deviation
^bpBV encodes Synechococcus sp. PCC7002 DesB for Δ15 desaturase (also known as ω3 or methyl end desaturase) and Synechococcus sp. PCC7002 Vipp1 for inducing thylakoid membranes; pDBV is the three-gene plasmid derived from pAM4418 (Fig. 2) developed in this work which includes the two genes of pBV plus Synechocystis sp. PCC6803 desD for Δ6 desaturase expression
^cdesA and desB were from Synechococcus sp. PCC7002
^dEstimated from mass if total fatty acid content is same as transformed strain
^edesD was from Synechocystis sp. PCC6803
^fdesB was from Synechocystis sp. PCC6803
^gdesB was from Synechocystis sp. PCC6803 plus a C-terminal FLAG tag
^hdesD was from Synechocystis sp. PCC6803 plus a C-terminal His tag

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