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Table 1 Sucrose yield in different cyanobacteria strains under co-culture conditions

From: Construction and analysis of an artificial consortium based on the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 to produce the platform chemical 3-hydroxypropionic acid from CO2

Host strains

Genotype

Cultural condition

Cultural medium

Titer (mg/L)

Productivity (mg/L/h)

Source of value

Synechococcus elongatus PCC 7942

NS3::Plac-cscB-Cmr

30 °C or 37 °C a, 2 % CO2, 65 μE m-2 s-1 light, 150 mM

NaCl for 2 days (baffled flasks)

CoYBG-11 or CoBBG-11b

156–625

3.25–13.02

[25]

NS3::Plac-cscB-Cmr

28 °C c, 1% CO2,

65 μmol m-2 s-1 with 16:8 h light/dark cycle 100 mM NaCl

For 4 days(baffled flasks)

BG-11 supplemented with 1 g/L HEPES (pH 8.9)

~210

~2.2

[22]

NS3::Plac-cscB-Cmr

35 °C, 2 % CO2, 65 μE m-2 s-1 light, 140 mM

NaCl for 3 days(bioreactor)

M1d

~250

~3.47

[21]

Synechococcus elongatus UTEX 2973

pJA-cscB

37 °C, 100 μE m−2 s−1 light, 150 mM NaCl for 3 days(100-ml round flask)

CoBG-11

295.5

~4.11

This study

pJA-cscB

30 °C, 100 μE m−2 s−1 light, 150 mM NaCl for 3 days(100-ml round flask)

CoBG-11

288

~4.00

This study

  1. aIn co-culture system, 37°C for E. coli and B. subtilis, and 30°C for S. cerevisiae
  2. bCoBBG-11 consists of BG-11 supplemented with 106 mM NaCl, 4 mM NH4Cl and 25 mM HEPPSO, pH-8.3 KOH. CoYBG-11 consists of BG-11 supplemented with 0.36 g/L yeast nitrogen base without amino acids (Sigma Aldrich), 106 mM NaCl, 25 mM HEPPSO, pH 8.3-KOH and 1 mM KPO3
  3. cIn co-culture system, 28°C for three yeast strains
  4. dM1 consists BG-11 medium was additionally supplemented with 15 mM NaNO3, 4.5 mM K2HPO4 (phosphate buffering), 1.5 mM MgSO4, 5 mM Na2SO4, 30 μM FeCl3, 30 μM Na2MoO4, and 1x additional trace metals before the addition of KOH to pH 8.3