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Table 1 Sucrose yield in different cyanobacteria strains under co-culture conditions

From: Construction and analysis of an artificial consortium based on the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 to produce the platform chemical 3-hydroxypropionic acid from CO2

Host strainsGenotypeCultural conditionCultural mediumTiter (mg/L)Productivity (mg/L/h)Source of value
Synechococcus elongatus PCC 7942NS3::Plac-cscB-Cmr30 °C or 37 °C a, 2 % CO2, 65 μE m-2 s-1 light, 150 mM
NaCl for 2 days (baffled flasks)
CoYBG-11 or CoBBG-11b156–6253.25–13.02[25]
NS3::Plac-cscB-Cmr28 °C c, 1% CO2,
65 μmol m-2 s-1 with 16:8 h light/dark cycle 100 mM NaCl
For 4 days(baffled flasks)
BG-11 supplemented with 1 g/L HEPES (pH 8.9)~210~2.2[22]
NS3::Plac-cscB-Cmr35 °C, 2 % CO2, 65 μE m-2 s-1 light, 140 mM
NaCl for 3 days(bioreactor)
Synechococcus elongatus UTEX 2973pJA-cscB37 °C, 100 μE m−2 s−1 light, 150 mM NaCl for 3 days(100-ml round flask)CoBG-11295.5~4.11This study
pJA-cscB30 °C, 100 μE m−2 s−1 light, 150 mM NaCl for 3 days(100-ml round flask)CoBG-11288~4.00This study
  1. aIn co-culture system, 37°C for E. coli and B. subtilis, and 30°C for S. cerevisiae
  2. bCoBBG-11 consists of BG-11 supplemented with 106 mM NaCl, 4 mM NH4Cl and 25 mM HEPPSO, pH-8.3 KOH. CoYBG-11 consists of BG-11 supplemented with 0.36 g/L yeast nitrogen base without amino acids (Sigma Aldrich), 106 mM NaCl, 25 mM HEPPSO, pH 8.3-KOH and 1 mM KPO3
  3. cIn co-culture system, 28°C for three yeast strains
  4. dM1 consists BG-11 medium was additionally supplemented with 15 mM NaNO3, 4.5 mM K2HPO4 (phosphate buffering), 1.5 mM MgSO4, 5 mM Na2SO4, 30 μM FeCl3, 30 μM Na2MoO4, and 1x additional trace metals before the addition of KOH to pH 8.3