Table 2 Significant LEfSe biomarkers with a linear discriminant analysis (LDA) score ≥ 4, using the respective substrate as class and the degree of overload (low, medium, high) as subclass

Cont

9

Candidatus Cloacimonas

Methanosaeta

Pedosphaeraceae_genus

Gracilibacter

8

Lachnospiraceae (uncultured genus)

Halocella

Tryp

6

Proteiniphilum

Desulfitobacterium

6

–

Tyr

6

Christensenellaceae_R-7_group

Lachnoclostridium_5

Treponema_2

Methanobacterium

6

Tepidimicrobium

Phe

6

–

6

–

ME

6

Paraclostridium

5

Sporanaerobacter

Proteiniphilum

Cas

6

Romboutsia

Methanoculleus

6

Gelria

Tepidanaerobacter