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Table 2 Initial ethanol and acetic acid concentrations, ethanol production and cell viability during repeated batches with encapsulated T18

From: Repeated batches as a strategy for high 2G ethanol production from undetoxified hemicellulose hydrolysate using immobilized cells of recombinant Saccharomyces cerevisiae in a fixed-bed reactor

 

Batch

1

2

3

4

5

6

7

8

9

10

A

 Cell Viability (%)

100

97

98

97

98

98

98

98

97

98

 [EtOH]0 (g/L)

0

5.0

4.8

6.5

6.6

5.9

5.6

5.5

6.0

5.8

 [HAc]0 (g/L)

0

0

0

0

0

0

0

0

0

0

 [EtOH] (g/L)

16.6

21.4

24.2

21.2

20.9

24.6

20.8

23.3

23.4

24.7

 QP (g/L/h)

4.2

4.1

4.7

4.2

4.1

4.7

4.3

4.5

4.4

4.6

B

 Cell viability (%)

99

98

98

98

97

98

98

97

96

92

 [EtOH]0 (g/L)

0

4.8

6.1

6.9

6.3

7.0

7.1

6.4

7.6

6.7

 [HAc]0 (g/L)

4.3

7.7

9.6

8.3

10.4

10.3

9.7

10.3

10.7

10.7

 [EtOH] (g/L)

16.1

22.7

23.8

25.0

24.6

25.8

25.6

26.8

27.5

27.8

 QP (g/L/h)

1.6

1.8

1.6

1.8

1.8

1.9

1.7

1.9

1.8

1.9

C

 Cell viability (%)

99

98

98

98

98

98

98

84

81

72

 [EtOH]0 (g/L)

0

4.1

6.0

6.1

5.9

5.8

5.8

5.4

6.0

6.1

 [HAc]0 (g/L)

7.9

11.7

13.3

13.2

14.0

14.0

14.4

14.2

14.4

14.8

 [EtOH] (g/L)

15.8

22.4

23.8

23.7

23.2

22.9

23.3

24.0

24.0

24.6

 QP (g/L/h)

1.6

1.7

1.6

1.8

1.4

1.4

1.5

1.5

1.3

1.6

D

 Cell viability (%)

99

98

98

98

98

98

90

80

66

61

 [EtOH]0 (g/L)

0

3.7

6.4

6.2

5.6

5.7

5.3

4.5

5.8

6.0

 [HAc]0 (g/L)

10.7

13.9

15.5

16.9

17.7

17.7

17.3

18.9

17.9

17.1

 [EtOH] (g/L)

14.2

15.9

15.2

15.9

15.5

15.5

15.3

16.7

13.7

14.7

 QP (g/L/h)

1.2

1.3

1.4

1.3

1.2

1.1

1.0

0.7

0.4

0.3

  1. Final cell viability and initial ethanol and acetic acid concentrations in repeated batch fermentations with encapsulated T18 in YPX 40 g/L with different acetic acid concentrations: A—0 g/L; B—4 g/L; C—8 g/L and D—10 g/L (35 °C, DO0 = 100, 50 gdrycells/L, 150 rpm and pH 5.2). Values are triplicates averages, with less than 5% standard error. [EtOH]0 and [HAc]0 correspond to initial accumulated ethanol and acetic acid concentrations, respectively; [EtOH] is the final ethanol concentration and QP is ethanol productivity