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Table 3 Strains and plasmids used in this study

From: Biosynthesis of acetylacetone inspired by its biodegradation

Strains or plasmidsGenotype/descriptionSource
Strains
 E. coli DH5FrecA endA1 Φ80dlacZΔM15 hsdR17(rKm+K)λInvitrogen
 E. coli BL21 (DE3)FompT hsdSB(rBmB) gal dcm λ (DE3)Invitrogen
 Q2837E. coli BL21(DE3) carrying pETDuet-Dke1 and pACYCDuet-MgsA-TpiAThis study
 Q3028E. coli BL21(DE3) carrying pETDuet-Tmo_Dke1This study
 Q3029E. coli BL21(DE3) carrying pETDuet-Pxe_Dke1This study
 Q3030E. coli BL21(DE3) carrying pETDuet-Dke1This study
 Q3080E. coli BL21(DE3) carrying pM1This study
 Q3081E. coli BL21(DE3) carrying pM2This study
 Q3082E. coli BL21(DE3) carrying pM3This study
 Q3085E. coli BL21(DE3) carrying pM6This study
 Q3086E. coli BL21(DE3) carrying pM7This study
 Q3148E. coli BL21(DE3) carrying pM9This study
 Q3149E. coli BL21(DE3) carrying pM10This study
 Q3150E.coli BL21(DE3) carrying pM11This study
 Q3151E. coli BL21(DE3) carrying pM12This study
 Q3152E. coli BL21(DE3) carrying pM13This study
 Q3153E. coli BL21(DE3) carrying pM14This study
 Q3154E. coli BL21(DE3) carrying pM15This study
 Q3155E. coli BL21(DE3) carrying pM16This study
 Q3170E. coli BL21(DE3) carrying pM17This study
Plasmids
 pETDuet-1AmpR, reppBR322, lacI PT7Novagen
 pACYCDuet-1CmR, p15A origin, lacI PT7Novagen
 pETDuet-Dke1pETDuet-1 harboring acetylacetone-cleaving enzyme (Dke1) from A. johnsoniiThis study
 pETDuet-Tmo_Dke1pETDuet-1 harboring acetylacetone-cleaving enzyme from T. mobilisThis study
 pETDuet-Pxe_Dke1pETDuet-1 harboring acetylacetone-cleaving enzyme from P. xenovoransThis study
 pACYCDuet-MgsA-TpiApACYCDuet-1 harboring methylglyoxal synthase (MgsA) and triose-phosphate isomerase (TpiA) from E. coliThis study
 pM1reppBR322 AmpRlacI PT7 Dke1K15QThis study
 pM2reppBR322 AmpRlacI PT7 Dke1S17DThis study
 pM3reppBR322 AmpRlacI PT7 Dke1Y21WThis study
 pM6reppBR322 AmpRlacI PT7 Dke1L103RThis study
 pM7reppBR322 AmpRlacI PT7 Dke1G105DThis study
 pM9reppBR322 AmpRlacI PT7 Dke1A60DThis study
 pM10reppBR322 AmpRlacI PT7 Dke1A60NThis study
 pM11reppBR322 AmpRlacI PT7 Dke1G101NThis study
 pM12reppBR322 AmpRlacI PT7 Dke1G101DThis study
 pM13reppBR322 AmpRlacI PT7 Dke1G101SThis study
 pM14reppBR322 AmpRlacI PT7 Dke1L103CThis study
 pM15reppBR322 AmpRlacI PT7 Dke1L103QThis study
 pM16reppBR322 AmpRlacI PT7 Dke1E140VThis study
 pM17reppBR322 AmpRlacI PT7 Dke1K15Q/A60DThis study