Table 3 Strains and plasmids used in this study
From: Biosynthesis of acetylacetone inspired by its biodegradation
Strains or plasmids | Genotype/description | Source |
|---|---|---|
Strains | ||
E. coli DH5 | F−recA endA1 Φ80dlacZΔM15 hsdR17(r−Km+K)λ− | Invitrogen |
E. coli BL21 (DE3) | F−ompT hsdSB(r−Bm−B) gal dcm λ (DE3) | Invitrogen |
Q2837 | E. coli BL21(DE3) carrying pETDuet-Dke1 and pACYCDuet-MgsA-TpiA | This study |
Q3028 | E. coli BL21(DE3) carrying pETDuet-Tmo_Dke1 | This study |
Q3029 | E. coli BL21(DE3) carrying pETDuet-Pxe_Dke1 | This study |
Q3030 | E. coli BL21(DE3) carrying pETDuet-Dke1 | This study |
Q3080 | E. coli BL21(DE3) carrying pM1 | This study |
Q3081 | E. coli BL21(DE3) carrying pM2 | This study |
Q3082 | E. coli BL21(DE3) carrying pM3 | This study |
Q3085 | E. coli BL21(DE3) carrying pM6 | This study |
Q3086 | E. coli BL21(DE3) carrying pM7 | This study |
Q3148 | E. coli BL21(DE3) carrying pM9 | This study |
Q3149 | E. coli BL21(DE3) carrying pM10 | This study |
Q3150 | E.coli BL21(DE3) carrying pM11 | This study |
Q3151 | E. coli BL21(DE3) carrying pM12 | This study |
Q3152 | E. coli BL21(DE3) carrying pM13 | This study |
Q3153 | E. coli BL21(DE3) carrying pM14 | This study |
Q3154 | E. coli BL21(DE3) carrying pM15 | This study |
Q3155 | E. coli BL21(DE3) carrying pM16 | This study |
Q3170 | E. coli BL21(DE3) carrying pM17 | This study |
Plasmids | ||
pETDuet-1 | AmpR, reppBR322, lacI PT7 | Novagen |
pACYCDuet-1 | CmR, p15A origin, lacI PT7 | Novagen |
pETDuet-Dke1 | pETDuet-1 harboring acetylacetone-cleaving enzyme (Dke1) from A. johnsonii | This study |
pETDuet-Tmo_Dke1 | pETDuet-1 harboring acetylacetone-cleaving enzyme from T. mobilis | This study |
pETDuet-Pxe_Dke1 | pETDuet-1 harboring acetylacetone-cleaving enzyme from P. xenovorans | This study |
pACYCDuet-MgsA-TpiA | pACYCDuet-1 harboring methylglyoxal synthase (MgsA) and triose-phosphate isomerase (TpiA) from E. coli | This study |
pM1 | reppBR322 AmpRlacI PT7 Dke1K15Q | This study |
pM2 | reppBR322 AmpRlacI PT7 Dke1S17D | This study |
pM3 | reppBR322 AmpRlacI PT7 Dke1Y21W | This study |
pM6 | reppBR322 AmpRlacI PT7 Dke1L103R | This study |
pM7 | reppBR322 AmpRlacI PT7 Dke1G105D | This study |
pM9 | reppBR322 AmpRlacI PT7 Dke1A60D | This study |
pM10 | reppBR322 AmpRlacI PT7 Dke1A60N | This study |
pM11 | reppBR322 AmpRlacI PT7 Dke1G101N | This study |
pM12 | reppBR322 AmpRlacI PT7 Dke1G101D | This study |
pM13 | reppBR322 AmpRlacI PT7 Dke1G101S | This study |
pM14 | reppBR322 AmpRlacI PT7 Dke1L103C | This study |
pM15 | reppBR322 AmpRlacI PT7 Dke1L103Q | This study |
pM16 | reppBR322 AmpRlacI PT7 Dke1E140V | This study |
pM17 | reppBR322 AmpRlacI PT7 Dke1K15Q/A60D | This study |