Fig. 2From: Overexpression of an endogenous type 2 diacylglycerol acyltransferase in the marine diatom Phaeodactylum tricornutum enhances lipid production and omega-3 long-chain polyunsaturated fatty acid contentFunctional characterisation of transgenic P. tricornutum lines coexpressing DGAT2B and OtElo5. a Fatty acid composition of WT and DGATElo5 cells in the S phase of growth in F/2 media. Each measurement is the average of three biological replicas (± standard error). b Neutral lipid content of DGATElo transgenic clones assessed by Nile Red fluorescence (refer to “Methods” ***section for experimental description). Relative level of fluorescence in the transformants compared to WT cells. Values are the average of three replicated experiments. Error bars indicate standard error. Fatty acids which are more abundant in transgenic cells compared to WT are indicated by an asterisk (*)Back to article page