Fig. 3

Cell growth and relative expression of transgenes in WT and transgenic P. tricornutum strains. a, b Cellular growth of WT and transgenic Pt_OtElo5, DGAT2B and DGATElo strains. Cells grown under N+ and N− conditions were harvested for lipid analysis where indicated. Values are the average of four experiments (± standard error). c, d The relative expression of OtElo5 and DGAT2B transcript levels analysed by qRT-PCR in transformants growing under N + and N− conditions. Algal cultures were grown under continuous light for 72 h in standard F/2 medium containing nitrogen (N +) or in N-deprived (N−) conditions. Relative transcript abundance was normalised to that of housekeeping gene RPS and calculated using the 2^−∆∆CT method. Values are the average of three replicated experiments. Error bars indicate standard error