Skip to main content
Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Metabolic engineering of a fast-growing cyanobacterium Synechococcus elongatus PCC 11801 for photoautotrophic production of succinic acid

Fig. 3

mRNA expression levels of the succinate pathway genes in selected strains. mRNA expression was observed in SA3, SA6 and SA7 strains in comparison to the wild-type for the exponential phase of growth (a) and late phase or stationary growth phase (b) of the cells. Primers amplifying approximately 200 bp amplicon were designed for each gene (Additional file 1: Table S5). RNA isolation and cDNA synthesis were performed by using commercially available kits and as per manufacturer’s protocols. The experiment was performed in duplicate and representative images of PCR products after gel electrophoresis are shown. 16S rRNA was used as a loading control. No reverse transcriptase control was treated as a negative control for each gene

Back to article page