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Table 1 List of the recombinant strains constructed in this study

From: Metabolic engineering of a fast-growing cyanobacterium Synechococcus elongatus PCC 11801 for photoautotrophic production of succinic acid

Strain

∆ NSI (∆DOP62_03525)a

∆glgA (∆DOP62_03790)a

∆ sdhB (∆DOP62_11515)a

Genes overexpressedb/knocked out

NSI

OgdA

SsaD

PEPC

glgA

gltA

SBPase

sdhB

YjjP

YjjB

WT

–

–

–

–

–

–

–

–

–

–

SA1

X

✓

✓

–

–

–

–

–

–

–

SA2

X

✓

✓

–

–

–

–

–

–

–

SA3

X

✓

✓

✓

–

–

–

–

–

–

SA4

X

✓

✓

✓

–

–

–

X

–

–

SA5

X

✓

✓

✓

X

–

–

–

–

–

SA6

X

✓

✓

✓

X

✓

–

–

–

–

SA7

X

✓

✓

✓

X

✓

✓

–

–

–

SA8

X

✓

✓

✓

X

✓

✓

X

–

–

SA9

X

✓

✓

✓

X

✓

✓

X

✓

✓

  1. Strains SA1-SA3 were constructed in S. elongatus PCC 7942 and PCC 11801 while the strains SA4-SA9 were constructed in PCC 11801 alone
  2. aLocus tags of the sites where constructs are integrated
  3. bThe locus tag/Uniprot identifiers as follows OgdA (Synpcc7002_A2770), SsaD (P25526), PEPC (Synpcc7942_2252), gltA (Synpcc7942_0612), Sbpase (Synpcc7942_0505), yjjP (POADD2) and yjjB (POADD5)
  4. X—knockout/disrupted site
  5. ✓—genes overexpressed
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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