Fig. 3

HPAEC elution patterns of oligosaccharide products after the incubation of tamarind seed xyloglucan (TXG) with NcLPMO9C (1.25 μM; a, b) and NcLPMO9M (1.25 μM; c, d) in the presence (1 mM; a, c) and absence (b, d) of ascorbic acid (Asc). TXG oligosaccharides released by xyloglucanase (XEG, 1.25 μM) in the presence of Asc (g) were added as the reference. In addition, TXG only (f), TXG with 1 mM Asc (e), TXG oligosaccharide standards (xyloglucan hepta + octa + nona saccharides; h) and a standard (i) containing a mixture of cellobiose, cellotriose, cellotetraose, cellopentaose and cellohexaose (from left to right in chromatogram) are shown