Fig. 5

HILIC-ESI-MS base-peak and extracted ion chromatograms of tamarind seed xyloglucan (TXG) digests from aNcLPMO9C and bNcLPMO9M. Extracted ion chromatograms are made for non-, C4- and C1-oxidized products (non-Ox, C4-Ox and C1-Ox) released by NcLPMO9C and NcLPMO9M (in the presence of 1 mM ascorbic acid). Determination of C4- and C1-oxidized TXG oligosaccharides is based on the m/z difference of − 2 and + 16, respectively, compared to m/z-values of corresponding non-oxidized oligosaccharides. The identification (numbered peaks) of C4-oxidized TXG oligosaccharides in NcLPMO9C- and NcLPMO9M-TXG-digest, based on the MS/MS fragmentation patterns, is shown in Tables 2 and 3, respectively. Asterisks indicate formic acid adducts of non-oxidized TXG oligosaccharides having the same m/z as C1-oxidized products