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Table 3 Strains and plasmids used in this study

From: Enhanced biohydrogen production from cotton stalk hydrolysate of Enterobacter cloacae WL1318 by overexpression of the formate hydrogen lyase activator gene

Strain or plasmid

Genotype and relevant characteristics

Reference or source

E. coli DH5α

F-, φ 80dlacZ ΔM15, Δ (lacZYA -argF) U169, deoR, recA1, endA1, hsdR17 (rK−, mK+), phoA, supE44, λ−, thi-1, gyrA96, relA1

Sangon

E. cloacae WL1318

Wild type

[26]

E. cloacae WL1318-fhlA

E. cloacae WL1318 containing pET28a-fhlA

This study

pUCm-T

TA Cloning vector, Ampr

Sangon

pET28a

Prokaryotic expression vector, Kanr

Miaolingbio

pET28a-fhlA

fhlA in EcoRI–XhoI sites of pET28a

This study