Fig. 1From: Purification and immobilization of engineered glucose dehydrogenase: a new approach to producing gluconic acid from breadwastea SDS-PAGE analysis of purified GDH. Lane 1 shows molecular size markers, and lane 2 is GDH purified by Ni–NTA affinity chromatography. b Size exclusion chromatography of GDH from lane 1 in panel (a). The blue trace represents the UV absorbance at 280 nm and the peak is indicative of the quantity of protein detected. The brown trace reflects the conductivity monitor which measures conductivity of buffer and samples for online monitoring of the true gradient. The green trace is applied if there is another buffer being used; since this sample was run in one buffer only, the trace has a value of 0Back to article page