Fig. 5

Detection of Scopoletin in F6H1 transgenic plants. Py-GC/MS was performed for inflorescence stem samples prepared from wild type and PkC4Hpro::F6H1 plants fully grown for 8 weeks and completely dried for 4 weeks without watering. a Total ion chromatogram of pyrolyzed products from wild type and PkC4Hpro::F6H1 lines. The triangle indicates the peak of scopoletin. b Mass spectra of pyrolyzed products of scopoletin. The upper and lower panels indicate mass spectra of scopoletin standard and pyrolyzed products, respectively, from the actual peak shown in (a). c Quantification of scopoletin content in AIR residue by Py-GC/MS. Boxplots represent the range of determined scopoletin content. Horizontal bars in the boxes indicate the median of the detected values. Upper and lower hinges of the boxes indicate 75% and 25% ranges of detected values, respectively. The upper and lower extreme bars of the boxplots indicate the maximum (upper) and the minimum (lower) of reported values, respectively. Each dot indicates raw scopoletin values in each F6H1 line