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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Biochemical characteristics and molecular mechanism of an exo-type alginate lyase VxAly7D and its use for the preparation of unsaturated monosaccharides

Fig. 1

Phylogenetic analysis and multiple amino acid sequence alignment of VxAly7D. a Phylogenetic analysis of VxAly7D. The phylogenetic tree was constructed with MEGA 7.0 using the neighbour-joining method. Bootstrap values were expressed based on 1000 replications, the numbers represent bootstrap values (confidence limits) representing the substitution frequencies per amino acid residue, and only values of 70% or above are shown at the nodes. The sequences from this study are shown in bold. b Multiple amino acid sequence alignment of VxAly7D with crystallized PL7 enzymes. The secondary structure elements shown above are referenced according to AlyA5. AlyA5, from Zobellia galactanivorans DsijT (CAZ98266.1); AlyA, from Klebsiella pneumoniae (AAA25049); PA1167, from Pseudomonas aeruginosa PAO1 (AAG04556); alyPG, from Corynebacterium sp. ALY-1 (BAA83339); AlgAT5 from Defluviitalea phaphyphila Alg1T (JWID00000000); FlAlyA from Flavobacterium sp. UMI-01 (BAP05660); A1-II’, from Sphingomonas sp. A1 (BAD16656); AlyA1, from Z. galactanivorans DsiJT (CAZ95239); AlyQ from Persicobacter sp. CCB-QB2 (WP_053404615)

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