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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Biochemical characteristics and molecular mechanism of an exo-type alginate lyase VxAly7D and its use for the preparation of unsaturated monosaccharides

Fig. 3

End products and minimal degradation substrate of recombinant VxAly7D. a TLC analysis of the end products of recombinant VxAly7D. Lane M, ΔDP2 and ΔDP3; Lane 1 and Lane 2 represent the end products of sodium alginate (3 mg) degraded by 20 U or 100 U recombinant VxAly7D, respectively. b The end products of sodium alginate were analysed by gel filtration chromatography. Action of 20 U recombinant VxAly7D on 3 mg sodium alginate at 30 °C for 16 h. The elution volumes were 17.09 ml for monosaccharides, 16.10 ml for disaccharides, and 14.90 ml for trisaccharides. c The end products of sodium alginate were analysed by negative ion ESI-MS. d Relative activities of recombinant VxAly7D towards M2–M5

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