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Fig. 7 | Biotechnology for Biofuels

Fig. 7

From: Biochemical characteristics and molecular mechanism of an exo-type alginate lyase VxAly7D and its use for the preparation of unsaturated monosaccharides

Fig. 7

Action mode of the W295A mutant. a The action mode was measured according to the changes in absorbance at 235 nm (dotted line) and the reduction in viscosity (solid line). One millilitre of enzyme (10 U) was added to 9 ml of substrate solution [0.3% (w/v) high-viscosity sodium alginate, 100 mM NaCl, 20 mM PB, optimal pH], followed by incubation at 30 °C for 0, 1, 5, 10, 30 and 60 min. b The time-course of sodium alginate degradation by the W295A mutant was determined by TLC. A total of 5 U of enzyme were added to 1 ml substrate solution [0.3% (w/v) sodium alginate, 100 mM NaCl, 20 mM PB, optimal pH], followed by incubation at 30 °C for 0 s, 10 s, 30 s, 1 min, 3 min, 5 min, 10 min, 20 min, 30 min, 40 min, or 60 min. c, d The time-course of polyM and polyG degradation by the W295A mutant were determined by TLC. A total of 3 U of enzyme were added to 1 ml substrate solution [0.3% (w/v) polyM or polyG, 100 mM NaCl, 20 mM PB, optimal pH], followed by incubation at 30 °C for 0 s, 10 s, 30 s, 1 min, 3 min, 5 min, 10 min, 20 min, 30 min, 40 min, or 60 min

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